A Learning Guide Flow cytometry is a technology that simultaneously measures and then analyzes multiple physical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. The properties measured include a particle’s relative size, relative granularity or internal complexity, and relative fluorescence intensity. These characteristics are determined using an optical-to-electronic coupling system that records how the cell or particle scatters incident laser light and emits fluorescence... Read more in PDF format...
Ian Storie Flow cytometry has become an essential tool in the research and clinical diagnostic laboratory. The range of available flow-based diagnostic tests and the number of worldwide laboratories that possess a flow cytometer are ever increasing. Results produced from such laboratories need to be as accurate and reliable as possible, since disease diagnosis, treatment and monitoring of treatment is dependent upon them. How can we be confident in our results and know that our results are comparable with other laboratories performing the same test? Clinical flow cytometrists must always bear in mind the sample they are testing comes from an actual person, and they owe it to that person to ensure the results are as accurate and reliable as possible. One solution is the establishment of methodological standardization both in-house and across laboratories, as well as the implementation of stringent internal and external quality assessment procedures. Research flow cytometrists can benefit from these procedures as well... Read more in PDF format...
Jodi Moore, Ph.D. April 2012 Goldenson 113 How to think about flow cytometry experiments…... Read more in PDF format...
Introduction Immunohistochemistry (IHC) combines histological, immunological and biochemical techniques for the identification of specific tissue components by means of a specific antigen/antibody reaction tagged with a visible label. IHC makes it possible to visualize the distribution and localization of specific cellular components within a cell or tissue... Read more in PDF format...
Bench philosophy (40): Eleven Flow Cytometry tips Though modern flow cytometers are becoming more and more user friendly and may even be operated by nonexperts, flow cytometry is still a tricky business. Ryan Duggan, Technical Director of the Flow Cytometry Core Facility at the University of Chicago (UCFlow) gives some advice. Read more in PDF format...